The aim of this work was to investigate the phytochemical screening, in-vitro anti-inflammatory activity, antioxidant and thrombolytic activities of crude methanolic extract of dry and fresh leaves of Ixora villosa (IxV). Chemical test revealed the presence of glycosides, steroids, tannins, flavonoids, reducing sugars, and amides in the plant extract. Anti-inflammatory and Antioxidant activities of the crude extract were determined by egg albumin denaturation and DPPH free radical scavenging method respectively with minor modification. The methanolic extract showed moderate anti-inflammatory potential at highest dose (500 µg/ml) used in the study. The test results were compared with the activity of positive control, Acetyl salicylic acid. The antioxidant activity test showed a gradual decrease in absorbance with increase in sample concentration. Significant IC50 value was obtained by the test extract (83.91µg/ml) in comparison with standard drug, Ascorbic acid (52.23µg/ml). During thrombolytic activity test, the test results of clot lysis were negligible in comparison with Streptokinase and distilled water used as positive control and negative control respectively. In conclusion, the methanolic extract from dry leaves of IxV could be used as potential source of new antioxidant and anti-inflammatory agents.
Keywords: Ixora villosa, anti-inflammatory, antioxidant, and thrombolytic.