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JOURNALS || ASIO Journal of Experimental Pharmacology & Clinical Research (ASIO-JEPCR) [ISSN: 2455-7080]
CHENOPODIUM ALBUM LINN. LEAVES PREVENT CARBON TETRACHLORIDE-INDUCED LIVER FIBROSIS IN RATS

Author Names : Manish M. Wanjari
Page No. : 16-22  volume 1 issue 1
Article Overview

ARTICLE DESCRIPTION: 

Manish M. Wanjari, Vanika Bajpai, Yadu Nandan Dey, Sudesh N. Gaidhani, Gajji Babu, Chenopodium Album Linn. Leaves prevent carbon tetrachloride-induced liver fibrosis in rats, ASIO Journal of Experimental Pharmacology & Clinical Research (ASIO-JEPCR), 2016, 1(1): 16-22.

ARTICLE TYPE: RESEARCH

dids no.: 03.2016-35559215,

dids Link:  http://dids.info/didslink/03.2016-25637255/


ABSTRACT:

The leaves of Chenopodium album Linn. are traditionally used in treatment of jaundice and spleen enlargement. The present work investigated the effect of aqueous extract of leaves of Chenopodium album on experimentally induced hepatotoxicity in rats to substantiate the traditional use in liver diseases. The hepatotoxicity was induced in rats by carbon tetrachloride (CCl4) treatment and in addition, vehicle or aqueous extract of the leaves of Chenopodium album (CAE) (100, 200 and 400 mg/kg) or silymarin (25 mg/kg) were administered daily orally for seven days. The hepatotoxicity was assessed by estimating the activities of marker enzymes and by histological studies. The oxidative stress was assessed by measuring lipid peroxidation and activities of superoxide dismutase and catalase in liver. The CAE treatment significantly prevented the CCl4-induced elevations the serum levels of glutmate oxaloacatate transaminase, pyruvate oxaloacatate transaminase, alkaline phosphatase, bilirubin, lactate dehydrogenase and triglycerides while decreased the total protein. Treatment with CAE attenuated the CCl4-induced fibrosis in liver tissue and also decreased elevated lipid peroxidation levels and inhibited decrease in activities of superoxide dismutase and catalase enzymes in liver. The effects of the CAE were comparable to that of standard antioxidant hepatoprotective agent, silymarin. These findings indicate the CAE exhibited hepatoprotective effect against CCl4-induced liver fibrosis which may be attributed to antioxidant action of CAE due to presence of antioxidant phytochemicals like flavonoids and other phenolic compounds. It indicates the therapeutic potential of Chenopodium album leaves and validates its traditional medicinal use in liver disorders.

Keywords: Bathua; Hepatotoxicity; Silymarin; Oxidative stress, Masson’s trichrome

 

Reference
  1. E. Hodgson, A textbook of modern toxicology. 3rd ed. (New Jersey: John Wiley & Sons, 2004).
  2. N. G. Ahmed, Hepatoprotective and anti hepatotoxic drugs from plant sources. 5th International Conference and Exhibition on Pharmaceutics & Novel Drug Delivery Systems. Pharm Anal Acta, 2015, 6,1.
  3. A. Subramoniam, P. Pushpangadan, Development of phytomedicine for liver diseases. Indian J Pharmacol, 1999, 31,166-175.
  4. M. Said, Hamdard Pharmacopoeia of Eastern Medicine. (Karachi: Hamdard National Foundation, 1969).
  5. N. Yadav, N. Vasudeva, S. Singh, S.K. Sharma, Medicinal properties of genus Chenopodium album: A review. Nat Prod Rad, 2007, 6, 131-134.
  6. K.R. Kirtikar, B.D. Basu, I.C.S. An, Indian Medicinal Plants, Vol 3, 2nd revised and enlarged ed. (Bishen Singh Mahendra pal Singh, Dehradun, 1975) 2072–2073.
  7. W. Bylka, Z. Kowalewski, Flavonoids in Chenopodium album L. and Chenopodium opulifolium L. (Chenopodiaceae) Herba Polonica, 1997, 43, 208-213.  
  8. J.A. Gonzalez, M. Gallardo, L.A. De Israilev, Leaf flavonoids in Chenopodium hircinum Schrad. and Chenopodium album L. (Chenopodiaceae). Phyton International Journal of Experimental Botany, 1998,  63, 279-281.
  9. K.M. Nadkarni, A.K. Nadkarni, Indian Material Medica. (Bombay: Popular Prakashan, 1982) 305.
  10. I.A. Saghir, A.A. Awan, S. Maji, M.A. Khan, S.J. Qureshi, S. Bano, Ethnopharamcological studied of Chikar and its allied areas of district Muzaffarabad. Online Journal of Biological Sciences, 2007, 1, 1165-1170.
  11. R.A. Qureshi, M. Ahmed, M.A. Ghufran, Indigenous knowledge of some important wild plants as a folk medicines in the area of Chhachh (Distt. Attock) Punjab, Pakistan. Elect J Environ Agri Food Chem, 2007, 6, 2500-2511.
  12. M.M.H. Siddiqui, A. Sultana, M.Y. Siddqui, Effect of Kabdeen in Warm-e-Kabid Vairoosi (viral hepatitis). Indian J Trad Know, 2005, 4, 416-420.
  13. R. Pundir, G. Singh, A.A. Pandey, S.A. Saraf, Demand of herbal hepatoprotective formulations in Lucknow- A survey. The Pharma Research, 2009, 1, 23-33.
  14. KR. Khandelwal, Practical pharmacognosy. 15th ed. (Pune: Nirali Prakashan, 2006) 149-156.
  15. Organization for Economic Co-operation and Development. Guideline for the testing of chemicals, 2000. Revised draft guideline 423. Document on acute oral toxicity and acute toxicity class method. (Source: www.oecd.org).
  16. S. Sengottuvelu, S. Duraisami, J. Nandhakumar, R. Duraisami, M. Vasudevan, Hepatoprotective activity of Camellia sinensis and its possible mechanism of action. Iranian J Pharmacol Ther, 2008, 7, 9-14.
  17. X. Ye, Y. Feng, Y. Tong, K.M. Ng, S. Tsao, G.K. Lau, C. Sze, Y. Zhang, J. Tang, J. Shen, S. Kobayashi, Hepatoprotective effects of Coptidis rhizoma aqueous extract on carbon tetrachloride-induced acute liver hepatotoxicity in rats. J Ethnopharmacol, 2009, 124, 130-136.
  18. A. Bindoli, L. Cavallini, N. Siliprandi, Inhibitory action of silymarin on lipid peroxide formation in rat liver mitochondria and microsomes. Biochem Pharmacol, 1977, 26, 2405-2409.
  19. P. Lettéron, G. Labbe, C. Degott, A. Berson, B. Fromenty, M. Delaforge, D. Larrey, D. Pessayre, Mechanism for the protective effects of silymarin against carbon tetrachloride-induced lipid peroxidation and hepatotoxicity in mice. Evidence that silymarin acts both as an inhibitor of metabolic activation and as a chain-breaking antioxidant. Biochem Pharmacol, 1990, 39, 2027-2034.
  20. D.A. Sorg, B. Buckner, A simple method of obtaining venous blood from small laboratory animals. Proc Soc Exp Biol Med, 1964, 115, 1131-1132.
  21. S. Reitman, S. Frankel, Method for determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol, 1957, 28, 56-63.
  22. P.R. Kind, E.J. King, Estimation of plasma phosphatase by determination of hydrolysed phenol with amino-antipyrine. J Clin Pathol, 1954, 7, 322-326.
  23. L. Jendrassik, P. Grof, Simplified photometric methods for the determination of the blood bilirubin. Biochemistry Z, 1938, 297, 81-89.
  24. C.P. Flack, J.W. Woollen, Prevention of interference by dextran with biuret-type assay of serum proteins. Clin Chem 1984, 30, 559-561.
  25. R.L. Searcy, Diagnostic Biochemistry. (New York: McGraw-Hill, 1969).
  26. E. Amador, L.E. Dorfman, W.E. Wacker, Serum lactic dehydrogenase activity: an analytical assessment of current assays. Clin Chem, 1963, 12, 391-399.
  27. H. Ohkawa, N. Ohishi, K. Yagi, Assay of lipid peroxidation in animal tissue by thiobarbituric acid reaction. Anal Biochem, 1979, 95, 351-358.
  28. S. Marklund, G. Marklund, Involvement of the superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry, 1974, 47, 469-474.
  29. H.  Aebi, Catalase. Methods Enzymol, 1984, 105, 121-126.
  30. Y. Dai, W.C. Ye, Z.T. Wang, H. Matsuda, M. Kubo, P.P.H. But, Antipruritic and antinociceptive effects of Chenopodium album L. in mice. J Ethnopharmacol, 2002, 81, 245-250.
  31. R.O. Recknagel, Carbon tetrachloride hepatotoxicity. Pharmacol Rev, 1967, 19, 145-208.
  32. G.L. Ploa, W.R. Hewitt, Detection and evaluation of chemically induced liver injury, in: Wallace Hayes, A., (Ed.), Principles and Methods of Toxicology, 2nd ed. (New York: Raven Press, 1989) 399–628.
  33. R. Ilavarasan, M. Vasudevan, S. Anbazhagan, S. Venkataraman, Antioxidant activity of Thespesia populnea bark extracts against carbon tetrachloride-induced liver injury in rats. J Ethnopharmacol, 2003, 87, 227-230.
  34. A.L. Tappel, Lipid peroxidation damage to cell components. Fed Proc, 1973, 32, 1870-1874.
  35. B.N. Ames, M.K. Shigenaga, T.M. Hagen, Oxidants, antioxidants, and the degenerative diseases of aging. Proc Natl Acad Sci, USA, 1993, 90, 7915-7922.
  36. J.M. McCord, Human disease, free radicals, and the oxidant/antioxidant balance. Clin Biochem, 1993, 26, 351-357.
  37. S.A. Tasaduq, K. Singh, S. Sethi, S.C. Sharma, K.L. Bedi, J. Singh, B.S. Jaggi, R.K. Johri, Hepatocurative and antioxidant profile of HP-1, a polyherbal phytomedicine. Human Exp Toxicol, 2003, 22, 639-645.
  38. P. Muriel, M.G. Moreno, Effects of silymarin and vitamins E and C on liver damage induced by prolonged biliary obstruction in the rat. Basic Clin Pharmacol Toxicol, 2004, 94, 99-104.
  39. U.S. Akula, B. Odhav, In vitro 5-Lipoxygenase inhibition of polyphenolic antioxidants from undomesticated plants of South Africa. J Med Plants Res, 2008, 2, 207-212.
  40. S. Kumar, D. Kumar, Antioxidant and free radical scavenging activities of edible weeds. Afr J Food, Agri Nutr Dev, 2009, 9, 1174-1190.
  41. P.M. Amália, M.N. Possa, M.C. Augusto, L.S. Francisca, Quercetin prevents oxidative stress in cirrhotic rats. Dig Dis Sci, 2007, 52, 2616-2621.
  42. Y.T. Chen, R.L. Zheng, Z.J. Jia, Y. Ju, Flavonoids as superoxide scavengers and antioxidants. Free Radic Biol Med, 1990, 9, 19-21.