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JOURNALS || ASIO Journal of Pharmaceutical & Herbal Medicines Research (ASIO-JPHMR) [ISSN: 2455-281X]

Author Names : Dhara Patel
Page No. : 01-04  Volume 4 Issue 1
Article Overview


Dhara Patel*, Khushboo Patel, Dhananjay Meshram, Prachi Patel, ASIO Journal of Pharmaceutical & Herbal Medicines Research (ASIO-JPHMR), Volume 4 (1); 2018; 01-04 


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In recent times, one of the social problems regarding world health is the stability of population growth. To control world population growth, male and female partners are equally responsible. Many methods have been devised for the female, whereas the male has not received enough attention in this respect. So, the necessity to develop a safe, effective and affordable precoital spermicidal contraceptive to control pregnancy and population growth still exists. The aim of this study was to highlight the work on extract of Parmelia Perlata lichen involved in male anti-fertility mechanism. The hypo-osmotic swelling (HOS) and the sperm viability test were used to detect the integrity of sperm membrane and vitality. The sperm revival test was also done to check the recovery of the sperm motility. Parmelia perlata methanolic extract 20 mg/ml concentration, induced complete immobilization of human spermatozoa and kill 100% spermatozoa within 20s in vitro. The sperm revival test did not show any spermatozoa that recovered their motilities. In the 20 mg/ml extract and N-9 treated groups, the rate of the normal HOS (swollen tails) and the viable sperms (unstained) was 0%, and the rate of the abnormal HOS (unswollen tails) and nonviable sperms (stained) was 100% indicating the plasma membrane degradation of the sperm. The current study indicates that methanolic lichen extract of Parmelia perlata possesses appreciable spermicidal potential, which may be explored as an effective vaginal contraceptive.

Keywords: Hypo-osmotic swelling, Sperm viability, Sperm membrane, Sperm motility, vaginal contraceptive, Parmelia Perlata.

  1. K. Chakrabart, S. Pal, AK. Bhattacharya. Sperm immobilization activity of Allium sativum L. and other plant extracts. Asian J An drol, 2003, 5, 131-135.
  2. A. Shafik. Prolactin injection, a new contraceptive method: experimental study. J Contraception, 1994, 50,191-9.
  3. VP. Kamboj, BN. Dhawan. Current status of plants investigated for fertility regulation in India. Korean Journal Pharmacy,1981; 12, 111.
  4. SK Garg, VS Mathur, RR Chaudhury.Screening of Indian Plants for antifertility activity. Indian J Exp Biol,  1978, 16(10), 1077-1079.
  5. K Santosh, N Satya. Herbal Remedies of Wetlands Macrophytes in India. International Journal of pharma and Biosciences, 2010, 1(2), 21-24.
  6. RR .Chaudhury, M Haq.  Review of Plants screened for antifertility activity-I. Bull Medico Ethno. Bot. Res, 1980a, 1(3), 408-419.
  7. VP Kamboj, Dhawan BN: Research on plants for fertility regulation in India. J Ethnopharmacol, 1982; 6:191-226.
  8. A.K.Sharma, M.C Sharma., M.P.Dobhal. Phytochemical investigation of therapeutic important lichen: Parmelia perlata. J. Nat. Prod. Plant Resour., 2012, 2 (1),101-106.
  9. D.A. Corley, K. Kerlikowske, R. Verma,  P Buffler. Protective association of aspirin/NSAIDS and esophageal cancer: A systemic review and meta analysis. Gastroenterology. 2003, 124, 47–56. 
  10. B. Thippeswamy, NR Sushma., KJ Naveenkumar. Antimicrobial property of bioactive factor isolated from Parmelia perlata. Int Multidiscip Res J.  2012, 2, 1-5.
  11. G  Sibi, V Apsara, K Dhananjaya, KR Ravikumar. and H Mallesha. Phytochemical and antibacterial properties of spices against food borne bacteria with special reference to Parmelia perlata. Global J. Bio-Sci. Biotechnol. 2013, 2, 145-149.
  12. E.C. Adikwu, D.O. Ibezim, G.N. Abonyi, D.O. Odaibo, Olaleye. In vitro evaluation of the antiviral activity of extracts from the lichen Parmelia perlata (L.) Ach. against three RNA viruses. J. Infect Dev Ctries. 2007, 1, 315-320.
  13. M.M. Kosanic, B.R. Rankovic, T.P. Stanojkovic. Antioxidant, antimicrobial and anticancer activities of three Parmelia species. J. Sci. Food Agric. 2012, 92, 1909-1916.
  14. Correia da Silva. Action of Parmelia caperata extracts on smooth muscle organs. Arch. Farmacol. Toxicol. 1976, 2, 143-152.
  15. S.B. Patil, V.A. Ghadyale, S.S. Taklikar, C.R. Kulkarni, A.U.   Arvindekar. Insulin secretagogue, alpha-glucosidase and antioxidant activity of some selected spices in streptozoto clinical induced diabetic rats. Plant Foods Hum Nutr. 2011, 66, 85-90.
  16. K Muller. Pharmaceutically relevant metabolites from lichens. Appl. Microbiol Biotechnol. 2001, 56, 9-16.
  17. S  Shailajan, M Joshi, B Tiwari. Hepatoprotective activity of Parmelia perlata (Huds.) Ach. against CCl4 induced liver toxicity in Albino Wistar rats.  J Appl Pharm Sci. 2014, 4(02), 70-74.
  18. World Health Organization. Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction. 4th ed.  Cambridge University Press, New York; 1999.
  19. FV Sander and SD Cramer. A practical method of testing the spermicidal action of chemical contraceptives. Human Fertil. 1941,6, 134–138.
  20. WD Ratnasooriya, AS Amarasekera, NS Perera and GA Premkumara,. Sperm anti-motility properties of a seed extract of  Abrus precatorius. Ethnopharmacol. 1991, 33, 85–90.
  21. RS Jeyendran.  HH Van der Ven. The hypoosmotic swelling test: an update. Arch Androl. 1992,  29, 105–116.
  22. RS Jeyendran, HH Van der Ven, M Perez-Pelaez, BG Crabo, and LJD Zaneveld,. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil. 1984, 70, 219–228.
  23. K. Gopalkrishnan,. Standardized procedures in human semen analysis. Curr Sci. 1995, 68, 353–362.