Dhara Patel*, Khushboo Patel, Dhananjay Meshram, Prachi Patel, ASIO Journal of Pharmaceutical & Herbal Medicines Research (ASIO-JPHMR), Volume 4 (1); 2018; 01-04
ARTICLE TYPE: Research
DOI Link :: http://doi-ds.org/doilink/10.2016-19146535/ ; DOI Link :: http://doi-ds.org/doilink/04.2018-39443618/
In recent times, one of the social problems regarding world health is the stability of population growth. To control world population growth, male and female partners are equally responsible. Many methods have been devised for the female, whereas the male has not received enough attention in this respect. So, the necessity to develop a safe, effective and affordable precoital spermicidal contraceptive to control pregnancy and population growth still exists. The aim of this study was to highlight the work on extract of Parmelia Perlata lichen involved in male anti-fertility mechanism. The hypo-osmotic swelling (HOS) and the sperm viability test were used to detect the integrity of sperm membrane and vitality. The sperm revival test was also done to check the recovery of the sperm motility. Parmelia perlata methanolic extract 20 mg/ml concentration, induced complete immobilization of human spermatozoa and kill 100% spermatozoa within 20s in vitro. The sperm revival test did not show any spermatozoa that recovered their motilities. In the 20 mg/ml extract and N-9 treated groups, the rate of the normal HOS (swollen tails) and the viable sperms (unstained) was 0%, and the rate of the abnormal HOS (unswollen tails) and nonviable sperms (stained) was 100% indicating the plasma membrane degradation of the sperm. The current study indicates that methanolic lichen extract of Parmelia perlata possesses appreciable spermicidal potential, which may be explored as an effective vaginal contraceptive.
Keywords: Hypo-osmotic swelling, Sperm viability, Sperm membrane, Sperm motility, vaginal contraceptive, Parmelia Perlata.
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